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GST pull-down is a classical in vitro protein interaction verification technique based on the high-affinity binding between Glutathione S-Transferase (GST) and Glutathione (GSH). A target protein is fused with GST and expressed, then immobilized onto GSH-coated magnetic beads or agarose resin to serve as the “bait”. Upon incubation with a lysate or purified protein containing candidate binding partners (the “prey”), specific interactions lead to capture and enrichment of the prey. After stringent washing to eliminate non-specific binding, the interacting proteins are identified via Western blot or mass spectrometry.