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Multi-Cargo Transfection reagents are designed for the simultaneous delivery of multiple nucleic acid cargos into the same cell. Supporting DNA/DNA co-transfection, DNA/RNA co-transfection, and multi-component genome editing systems, the reagents deliver balanced cargo expression, high reproducibility, and outstanding transfection efficiency. These reagents are ideally suited for reporter assays, viral packaging, CRISPR workflows, CAR-T engineering, and other complex gene delivery applications.