Filtered Products

Streptavidin-Biotin pull-down is an in vitro target fishing technique built upon the ultra-high affinity non-covalent interaction between Streptavidin and Biotin. Biotin serves as a small-molecule labeling probe, chemically conjugated to candidate drug molecules, metabolites, or ligands to form a “biotinylated bait”. After incubation with cell lysates, the bait is captured using streptavidin immobilized on agarose beads or magnetic beads. Given the extraordinary binding strength and stability, the complex remains intact even under stringent washing conditions (high salt, detergents, extreme pH), enabling efficient enrichment and identification of low-abundance interacting proteins.