UcallM® longRNA Ampli Kit

UcallM® longRNA-seq Kit enables efficient and uniform amplification for low-input RNA samples (including mRNA, lncRNA, etc.). Powered by optimized in-vitro transcription technology, it achieves robust amplification starting from as low as 1 ng input RNA while minimizing amplification bias. It serves as an ideal pre-amplification tool for NGS, gene detection and molecular cloning. Kit Specifications

Experimental Workflow

Key Advantages 1.Ultra-low input: starting material down to 1 ng RNA 2.Optimized linear-amplification strategy to mitigate amplification bias 3.Effective amplification for full-length / long-fragment transcripts Applications 1.Amplify trace RNA derived from single cells or small cell populations to generate high-quality template for high-depth whole-transcriptome NGS 2.Process precious and degraded RNA specimens for disease diagnosis, molecular subtyping and gene-expression profiling 3.Support high-coverage detection of low-abundance transcripts, e.g. tumor-mutation screening, fusion-gene discovery and novel-transcript identification

Customer Publications 1.Human Reproduction. A predictive model based on seminal-plasma exosomal lncRNAs for forecasting intratesticular sperm presence in non-obstructive azoospermia (DOI:10.1093/humrep/deaa184).

Fig. 1-1. The UcallM® longRNA Ampli Kit was used for cDNA generation and amplification, followed by qRT-PCR analysis of candidate lncRNAs. 2.J Cell Physiol. Altered circulating exosomal circRNA profiles in schizophrenia: novel biomarkers and mechanistic insights (DOI:10.1002/jcp.29873).

Fig. 1-2. This study synthesized cRNA from exosomal total RNA with UcallM® longRNA Ampli Kit for downstream qRT-PCR validation of circRNA expression.