UcallM® Small RNA Library Fast Kit

UcallM® Small RNA Library Fast Kit is a rapid library preparation kit designed for non-coding small RNA research. It adopts an innovative ligation system and optimized reaction procedure to achieve low bias. Featuring a streamlined experimental workflow and short hands-on time, it greatly improves experimental efficiency and delivers high library yield even with low input material. This kit is used for profiling miRNA and other small RNAs. It serves as a practical tool for exploring gene regulatory mechanisms in basic research, discovering disease biomarkers and screening drug targets in translational medicine, as well as studies in epigenetics and other fields.

Experimental Workflow

Key Highlights 1.Rapid library construction with short experimental turnaround time 2.Simplified workflow and easy operation 3.Excellent consistency and low bias Applications 1.Gene expression quantification and differential expression analysis 2.Discovery of disease biomarkers and molecular subtyping 3.Functional genomics and molecular mechanism research

Empirical Data

Figure 1-4. Venn diagram showing the intersection of small RNA target genes analyzed by different software (Miranda & RNAhybrid)

Figure 1-5. Heatmap of significantly differentially expressed small RNAs Customer Publication

1. Int J Biol Sci. Activated microglial exosomes promote retinal angiogenesis via the Irf1/miR-155-5p/Socs1 axis (DOI: 10.7150/ijbs.79784)

Figure 1-6. Differential expression analysis of exosomal miRNAs.

Figure 1-7. Prediction and validation of miRNA target genes.

2. Front Cell Infect Microbiol*. Plasma-derived exosomal sncRNAs serve as diagnostic biomarkers for the early detection of HBV-associated acute-on-chronic liver failure (DOI:10.3389/fcimb.2022.923300)

Figure 1-8. In this study, small RNA-seq was used to comprehensively profile various sncRNAs in plasma exosomes; the expression profiles of sncRNAs under different disease states (normal, chronic hepatitis B flare, HBV-ACLF) were analyzed.