UcallM® mini longRNA-seq Kit

UcallM® mini longRNA-seq Kit is designed for whole-transcriptome library construction with trace amounts of RNA samples, including antibody-enriched modified RNA fragments (e.g., meRIP, acRIP). It employs strand-specific library construction technology, and optimized buffer systems to preserve RNA integrity. The streamlined workflow enables rapid RNA library construction and subsequent sequencing. Compatible with RNA modification IP kits, it supports profiling of RNA modification landscapes. Widely applied in epitranscriptomics, tumor pathogenesis, therapeutic target discovery and other research fields.

Note: For RNA modification immunoprecipitation kits, please refer to the epitranscriptomics section. Experimental Workflow

Key Highlights 1.Low RNA input requirement (as low as 1 ng), compatible with FFPE samples 2.Efficient rRNA depletion probes to guarantee effective data yield 3.Optimized system, conditions and workflow for easy operation

Customer Publications 1.Cell Mol Immunol. A novel stromal protein TINAGL1 promotes bacterial colonization and gastritis during Helicobacter pylori infection (DOI: 10.1038/s41423-023-01055-4)

Figure 1-9. UcallM® mini longRNA-seq Kit was used to construct strand-specific RNA libraries for transcriptome sequencing. 2.Adv Sci. FUS mediates selective packaging of circRNAs into small extracellular vesicles via stress granules in oxygen-deprived neurons (DOI:10.1002/advs.202404822)

Figure 1-10. UcallM® mini longRNA-seq Kit was utilized for library preparation in RICK-seq experiments.