UcallM® ssDNA Library Prep Kit

UcallM® ssDNA Library Prep Kit is optimized for trace and degraded nucleic-acid specimens. It uses proprietary single-strand-ligation chemistry to maximally preserve original molecular information and completes full library-building within 3 hours per sample, yielding high-uniformity libraries with low duplicate-read rates. Compatible with ssDNA, dsDNA and mixed-input samples. Suitable for WGS, cfDNA-seq and library construction from FFPE-derived DNA.

Experimental Workflow

Key Advantages 1.High sensitivity and high library yield 2.Streamlined workflow; single-sample library-building finished in 3 hours 3.Broad-sample compatibility; supports multiple DNA-sequencing workflows (WGS, cfDNA-seq etc.)

Customer Publications 1.Adv Sci. NSUN5 drives hepatocellular-carcinoma progression and represents a potential therapeutic target (DOI:10.1002/advs.202404083).

Figure 1-41. In this study, the single-stranded DNA library prep kit was used for library construction of ChIP-seq experiments. 2.Adv Sci. 65K/RNPC3 interacts with ANKRD11 to modulate histone deacetylation (DOI:10.1002/advs.202307804). This kit was applied for ChIP-seq library-preparation in both studies.

Figure 1-42. This article also adopted the single-stranded DNA library prep kit to prepare libraries for ChIP-seq experiments.