UcallM® Hi-C Library Prep Kit

UcallM® Hi-C Library Prep Kit is dedicated to whole-genome chromatin-interaction profiling. Hi-C (High-throughput Chromosome Capture) captures genome-wide DNA-DNA contacts and generates high-resolution 3D-chromatin maps, resolving A/B compartments, topologically-associating domains (TADs) and chromatin loops. This kit features heavily-optimized restriction-enzyme efficiency, ligation specificity and library-complexity control for improved data resolution and reliability. Experimental workflows have been streamlined to shorten hands-on time. It serves as a powerful tool for epigenetics and spatial-interactome research, investigating 3D-genome-mediated gene regulation and disease-associated structural variants.

Experimental Workflow

Applications 1.Construct high-resolution 3D-genome maps and characterize inter-chromatin-fragment interactions 2.Decipher 3D-genome-driven gene-regulation mechanisms (enhancer-promoter contacts, cell-fate determination); integrate with RNA-seq / ChIP-seq multi-omics 3.Investigate pathogenic structural-variants in human diseases and mine potential biomarkers & therapeutic targets

Customer Publication Cell Rep Med. DDX5 super-enhancer drives nasopharyngeal-carcinoma vascular-mimicry and metastasis via up-regulating ADAM10 transcription (DOI:10.1016/j.xcrm.2025.102146). This study uncovered the key mechanism underlying the resistance of nasopharyngeal carcinoma to anti-angiogenic drugs (AADs). The research team found that during anti-angiogenic treatment, tumors establish compensatory new blood supply systems by forming vasculogenic mimicry, which leads to therapeutic resistance.

Figure 1-43. In this study, Epibiotech Hi-C Library Prep Kit was applied for library preparation of Hi-C experiments.