- Molecule
- IL36A/IL-1F6
- Background
- Interleukin-36 alpha (IL-36α) is an 18 kDa cytokine with 154 amino acid residues. IL-36α is one of the IL-36 agonists secreted from keratinocytes. When IL-36α binds to the IL-36 receptor, it activates NF-κB and MAPK signaling pathways that induce inflammatory responses. In addition, it also stimulates the production of inflammatory cytokines, such as IFN-γ, TNF-α, and IL-6.
- Species
- Human
- Source
- E. coli
- Tags
- C-6His
- Accession
- Q9UHA7
- KnownAs
- IL36A, Interleukin-1 family member 6 (IL-1F6), FIL1ε (FIL1E), Interleukin-1ε (IL1E)
- Protein Length
- Lys6-Phe158
- Predicted Molecular Mass
- 18.05 kDa
- N-terminal Sequence
- --
- SDS-PAGE
- 17 kDa, reducing condition
- Endotoxin
- <0.1 EU/µg as determined by LAL test.
- Concentration
- --
- Purity-SDS-PAGE
- >98% as determined by SDS-PAGE.
- Purity-SEC-HPLC
- --
- Activity
- --
- Form
- Lyophilized
- Formulation
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
- Shipping
- The product is shipped on dry ice/polar packs.
Upon receipt, store it immediately at the temperature listed below.
- Storage
- Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3-6 months.
Please minimize freeze-thaw cycles and use a manual defrost freezer.
- Reconstitution
- Reconstitute the lyophilized protein in sterile water at a concentration of greater than 100 µg/mL, and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.