- PackSize
- 50ug/100ug
- Target
- oxLDL
- Reactivity
- Human
- Applications
- ELISA, Bioactivity: FACS, Functional assay, Research in vivo
- Host Species
- Human
- Isotype
- IgG1-lambda
- Conjugate/Modification
- --
- Recommended Dilution Ratio
- --
- Composition
- 0.01M PBS, pH 7.4.
- Purification Process
- Protein A/G purified from cell culture supernatant.
- Purity
- >95% purity as determined by SDS-PAGE.
- storageCondition
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
- Clonality
- Monoclonal
- Clone Number
- --
- Immunogen
- Mammalian Cells
- Alias
- oxLDL
- Species
- --
- Gene ID-1
- --
- Uniprot
- --
- Background
- Adenosine 3', 5'-cyclic monophosphate (cAMP) is a key second messenger molecule in intracellular signal transduction. Monitoring cAMP levels is one of the most common ways to screen for agonists and antagonists of G protein–coupled receptors(GPCRs). Bioyeargene cAMP ELISA Kit is based on the competition between HRP-labeled cAMP and free cAMP for fixed amounts of mouse monoclonal anti-cAMP antibody(cAMP MAb) binding sites. Because the concentration of the HRP-labeled cAMP is held constant while the concentration of cAMP varies, the amount of HRP-labeled cAMP that is able to bind to the cAMP MAb will be inversely proportional to the concentration of cAMP in the well. This antigen-antibody complex binds to the goat anti-mouse IgG that has been previously attached to the well. The plate is washed to remove any unbound reagents and then developed. The ELISA typically displays an IC50(50% B/B0) of approximately 12 pmol/mL and a detection limit of approximately 0.15 pmol/mL.<br>• Sphingosine 1-phosphate: Lipid signaling in pathology and therapy., PMID:31624181<br/>• Sphingosine-1-phosphate (S1P) in ovarian physiology and disease., PMID:31455516<br/>• Sphingosine-1-phosphate (S1P) receptors: Promising drug targets for treating bone-related diseases., PMID:32155312<br/>• Pancreatic Acinar Cells-Derived Sphingosine-1-Phosphate Contributes to Fibrosis of Chronic Pancreatitis via Inducing Autophagy and Activation of Pancreatic Stellate Cells., PMID:37634735<br/>• Significance of sphingosine-1-phosphate in cardiovascular physiology and pathology., PMID:32278039<br/>• Sphingosine 1-Phosphate (S1P)/ S1P Receptor Signaling and Mechanotransduction: Implications for Intrinsic Tissue Repair/Regeneration., PMID:31703256<br/>• Sphingosine 1-phosphate (S1P) receptor modulators as an induction and maintenance therapy for ulcerative colitis: a systematic review and meta-analysis of randomized controlled trials., PMID:38153524<br/>• S1P (Sphingosine-1-Phosphate)-Induced Vasodilation in Human Resistance Arterioles During Health and Disease., PMID:36070401<br/>• Sphingosine 1-phosphate (S1P) signalling: Role in bone biology and potential therapeutic target for bone repair., PMID:28855094<br/>• Sphingosine 1-phosphate signalling., PMID:24346695<br/>
- Tissue Expression
- --
- Signaling_pathway
- --
- Research Areas
- --