- Target
- Histone H3.1
- Reactivity
- Human
- Applications
- ICFC,WB,ChIP,Direct ELISA, ICC,
- MW(Calculated)
- --
- Host Species
- Rat
- Isotype
- Rat IgG2a, κ
- Recommended Dilution Ratio
- Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis . For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For ChIP applications, the suggested dilution for ChIP application is 1:50-1:100 by volume. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Purification
- The antibody was purified by affinity chromatography.
- Storage
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Concentration
- 0.5 mg/mL
- Clonality
- Monoclonal
- Clone Number
- 5D10D4
- Immunogen
- Histone H3.1 S28ph peptide (21-39)(ATKAARK(phS)APATGGVKKPH), Freund's complete adjuvant.
- Specificity
- Histone
- Gene Name
- --
- Other Name
- Histone-H3
- Gene ID
- 8350
- Cellular Localization
- Phospho-Proteins
- Function
- H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Therefore, histones limit the accessibility of DNA by providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.