U-Blot® XBP-1 Monoclonal Antibody

Name: U-Blot® XBP-1 Monoclonal Antibody
SKU: WM0467
Availability: InStock

$268.00

Size:50μL

SKU: WM0467-50

Catalog No.: WM0467

Specification: 50μL/100μL

Stock: In stock

U-Blot® XBP-1 Monoclonal Antibody Learn more

Product details Specifications Q & A Education

Background：This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively e

Product Overview

Catalog No.: WM0467
SKU: WM0467-50
Category: Mouse mAbs
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: XBP-1
Reactivity: Human
Application: WB,ELISA
MW(Calculated): 29kD
MW(Observed): --
Host Species: Mouse
Isotype: --
Conjugate/Modification: Unmodified
Modification site: --
Recommended Dilution Ratio: WB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications.
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: Affinity purification
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: --
Clonality: Monoclonal
Clone No.: --
Immunogen: Purified recombinant fragment of human XBP-1 expressed in E. Coli.
Sequence: --
Specificity: XBP-1 Monoclonal Antibody detects endogenous levels of XBP-1 protein.
Gene Name: XBP1
Protein Name: X-box-binding protein 1
Other Name: XBP1;TREB5;XBP2;X-box-binding protein 1;XBP-1;Tax-responsive element-binding protein 5
Organism-1: Human
Gene ID-1: 7494
SwissProt-1: P17861
Organism-2: Mouse
Gene ID-2: --
SwissProt-2: O35426
Organism-3: --
Gene ID-3: --
SwissProt-3: --
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Endoplasmic reticulum . Colocalizes with ERN1 and KDR in the endoplasmic reticulum in endothelial cells in a vascular endothelial growth factor (VEGF)-dependent manner (PubMed:23529610). .; [Isoform 1]: Nucleus . Cytoplasm . Endoplasmic reticulum membrane ; Single-pass type II membrane protein . Endoplasmic reticulum membrane ; Peripheral membrane protein . Membrane ; Peripheral membrane protein . Shows no preferential localization to either the nucleus or the cytoplasm (By similarity). Shuttles between the nucleus and the cytoplasm in a CRM1-dependent manner (PubMed:16461360). Localizes predominantly at the endoplasmic reticulum membrane as a membrane-spanning protein; whereas may be only marginally localized on the cytosolic side of the ER membrane as a peripheral membrane (PubMed:19394296, PubMed:25190803). .; [Isoform 2]: Nucleus . Cytoplasm . Localizes predominantly in the nucleus. Colocalizes in the nucleus with SIRT1. Translocates into the nucleus in a PIK3R-, ER stress-induced- and/or insulin-dependent manner (By similarity). .; [X-box-binding protein 1, cytoplasmic form]: Cytoplasm . Nucleus . Localizes in the cytoplasm and nucleus after HM13/SPP-mediated intramembranaire proteolytic cleavage of isoform 1 (PubMed:25239945). .