U-Blot® PCNA Monoclonal Antibody(12D10), Biotin Conjugated

Name: U-Blot® PCNA Monoclonal Antibody(12D10), Biotin Conjugated
SKU: WM0890
Availability: InStock

$268.00

Size:50μL

SKU: WM0890-50

Catalog No.: WM0890

Specification: 50μL/100μL

Stock: In stock

U-Blot® PCNA Monoclonal Antibody(12D10), Biotin Conjugated Learn more

Product details Specifications Q & A Education

Background：The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008],

Product Overview

Catalog No.: WM0890
SKU: WM0890-50
Category: Mouse mAbs
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Research Use Only: Yes

Additional Specifications

Target: PCNA
Reactivity: Human,Rat,Mouse
Application: WB,IF,IHC
MW(Calculated): --
MW(Observed): 30-33kD
Host Species: Mouse
Isotype: IgG1
Conjugate/Modification: Biotin
Modification site: Unmodified
Recommended Dilution Ratio: Optimal working dilutions should be determined experimentally by the investigator;Suggested starting dilutions are as follows:WB 1:5000;IHC 1:200.
Form: Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative and 50% Glycerol.
Source: --
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Purity: --
storageCondition: Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Concentration: 1mg/ml
Clonality: Monoclonal
Clone No.: 12D10
Immunogen: --
Sequence: --
Specificity: PCNA Monoclonal Antibody(12D10) Biotin conjugated specially designed for your WB or IHC analysis.
Gene Name: PCNA
Protein Name: Proliferating cell nuclear antigen
Other Name: PCNA;Proliferating cell nuclear antigen;PCNA;Cyclin
Organism-1: Human
Gene ID-1: 5111
SwissProt-1: P12004
Organism-2: --
Gene ID-2: --
SwissProt-2: --
Organism-3: --
Gene ID-3: --
SwissProt-3: --
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Nucleus . Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase (PubMed:15543136). Co-localizes with SMARCA5/SNF2H and BAZ1B/WSTF at replication foci during S phase (PubMed:15543136). Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. .

Tech Support

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Documents

Datasheet, COA, SDS, and protocol files can be requested from technical support.

Storage & Handling

Storage: Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.