U-Blot® DDDDK-Tag(binds to flag sequnence) mouse mAb(12M11)

U-Blot® DDDDK-Tag(binds to flag sequnence) mouse mAb(12M11)
U-Blot® DDDDK-Tag(binds to flag sequnence) mouse mAb(12M11)
$268.00
Size:50μL
SKU: WM1231-50

Catalog No.: WM1231

Specification: 50μL/100μL

Stock: In stock

U-Blot® DDDDK-Tag(binds to flag sequnence) mouse mAb(12M11) Learn more

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Product information

Product detailsSpecificationsQ & AEducation

Background：The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

Product Overview

Catalog No.
WM1231
SKU
WM1231-50
Category
Mouse mAbs
Product Type
Other
Size
50μL/100μL
Stock Status
In stock
Available Stock
20
Minimum Order Quantity
1

Storage & Compliance

Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only
Yes

Additional Specifications

Target
Flag-tag
Reactivity
Species independent
Application
WB,ELISA,IP,IF,CoIP
MW(Calculated)
--
MW(Observed)
--
Host Species
Mouse
Isotype
VH IgG1 and VL Kappa
Conjugate/Modification
Unmodified
Modification site
--
Recommended Dilution Ratio
WB 1:10000-50000;ELISA 1:20000-50000;IP 1:50-1:200;IF 1:1000 -3000
Form
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Source
Mouse VH IgG1 and VL Kappa was expressed from 293F cells
Purification
Protein A purified
Purity
>95% SDS-PAGE
storageCondition
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
--
Clonality
Monoclonal
Clone No.
12M11
Immunogen
Synthetic Peptide of Flag-Tag
Sequence
--
Specificity
This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library immunized with DDDK peptide. Constant region of this antibody is mouse IgG1 and mouse kappa.
Gene Name
Flag tag; Flag-tag,DDDDK TAG, DDDDK-TAG, DYKDDDDK tag,DYKDDDDK-tag
Protein Name
--
Other Name
--
Organism-1
--
Gene ID-1
--
SwissProt-1
--
Organism-2
--
Gene ID-2
--
SwissProt-2
--
Organism-3
--
Gene ID-3
--
SwissProt-3
--
Organism-4
--
gene ID-4
--
SwissProt-4
--
Cellular Localization
--

Tech Support

ucallm

01

Documents

Datasheet, COA, SDS, and protocol files can be requested from technical support.

02

Storage & Handling

Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
06

Contact Support

Emailinfo@mail.ucallm.com

Phone+(1)-866-986-9598

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HoursMonday-Friday 09:00-18:00 CST

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Keywords:Mouse mAbs