U-Blot® DDDDK-Tag(binds to flag sequnence) mouse mAb(12M11)

Name: U-Blot® DDDDK-Tag(binds to flag sequnence) mouse mAb(12M11)
SKU: WM1231
Availability: InStock

$268.00

Size:50μL

SKU: WM1231-50

Catalog No.: WM1231

Specification: 50μL/100μL

Stock: In stock

U-Blot® DDDDK-Tag(binds to flag sequnence) mouse mAb(12M11) Learn more

Product details Specifications Q & A Education

Background：The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

Product Overview

Catalog No.: WM1231
SKU: WM1231-50
Category: Mouse mAbs
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: Flag-tag
Reactivity: Species independent
Application: WB,ELISA,IP,IF,CoIP
MW(Calculated): --
MW(Observed): --
Host Species: Mouse
Isotype: VH IgG1 and VL Kappa
Conjugate/Modification: Unmodified
Modification site: --
Recommended Dilution Ratio: WB 1:10000-50000;ELISA 1:20000-50000;IP 1:50-1:200;IF 1:1000 -3000
Form: PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Source: Mouse VH IgG1 and VL Kappa was expressed from 293F cells
Purification: Protein A purified
Purity: >95% SDS-PAGE
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: --
Clonality: Monoclonal
Clone No.: 12M11
Immunogen: Synthetic Peptide of Flag-Tag
Sequence: --
Specificity: This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library immunized with DDDK peptide. Constant region of this antibody is mouse IgG1 and mouse kappa.
Gene Name: Flag tag; Flag-tag,DDDDK TAG, DDDDK-TAG, DYKDDDDK tag,DYKDDDDK-tag
Protein Name: --
Other Name: --
Organism-1: --
Gene ID-1: --
SwissProt-1: --
Organism-2: --
Gene ID-2: --
SwissProt-2: --
Organism-3: --
Gene ID-3: --
SwissProt-3: --
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: --