U-Blot® Phospho Smad3(Ser425) mouse Monoclonal Antibody(1D9)

Name: U-Blot® Phospho Smad3(Ser425) mouse Monoclonal Antibody(1D9)
SKU: WM1773
Availability: InStock

$288.00

Size:50μL

SKU: WM1773-50

Catalog No.: WM1773

Specification: 50μL/100μL

Stock: In stock

U-Blot® Phospho Smad3(Ser425) mouse Monoclonal Antibody(1D9) Learn more

Product details Specifications Q & A Education

Background：The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009],

Product Overview

Catalog No.: WM1773
SKU: WM1773-50
Category: Mouse mAbs
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: Smad3
Reactivity: Human,Mouse,Rat
Application: IHC,IF
MW(Calculated): --
MW(Observed): 52kD
Host Species: Mouse
Isotype: --
Conjugate/Modification: Phospho
Modification site: Ser425
Recommended Dilution Ratio: IHC 1:50-300;IF 1:50-200
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Monoclonal
Clone No.: 1D9
Immunogen: Synthetic Peptide of Phospho-Smad3(S425)
Sequence: --
Specificity: The antibody detects endogenous Phospho-Smad3(S425) protein
Gene Name: SMAD3 MADH3
Protein Name: Mothers against decapentaplegic homolog 3 (MAD homolog 3) (Mad3) (Mothers against DPP homolog 3) (hMAD-3) (JV15-2) (SMAD family member 3) (SMAD 3) (Smad3) (hSMAD3)
Other Name: SMAD3;MADH3;Mothers against decapentaplegic homolog 3;MAD homolog 3;Mad3;Mothers against DPP homolog 3;hMAD-3;JV15-2;SMAD family member 3;SMAD 3;Smad3;hSMAD3
Organism-1: Human
Gene ID-1: 4088
SwissProt-1: P84022
Organism-2: Mouse
Gene ID-2: --
SwissProt-2: Q8BUN5
Organism-3: Rat
Gene ID-3: --
SwissProt-3: P84025
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Cytoplasm . Nucleus . Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969, PubMed:21145499). Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15601644). MAPK-mediated phosphorylation appears to have no effect on nuclear import (PubMed:19218245). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236). Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm of the inner cell mass at the blastocyst stage (By similarity). .