- Target
- Oct4
- Reactivity
- Human, Mouse
- Applications
- WB,ICC, ChIP,
- MW(Calculated)
- --
- Host Species
- Mouse
- Isotype
- Mouse IgG2b, κ
- Recommended Dilution Ratio
- Each lot of this antibody is quality control tested by Western blotting . Western blotting, suggested working dilution(s): Use 0.5 µg/ml antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration of 2.5 µg/ml is recommended. For ChIP applications, the suggested use of this reagent is 1:50 to 1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Previous lots of this product may have been formulated with 0.1% or 0.05% NaN 3 instead of 0.09% NaN 3 . For further information please contact BioLegend Technical Support or Customer Service .
- Purification
- --
- Storage
- Upon receipt, store undiluted between 2°C and 8°C.
- Concentration
- 0.5 mg/ml
- Clonality
- Monoclonal
- Clone Number
- RMM2A20
- Immunogen
- Partial human Oct4 recombinant protein (1-141aa).
- Specificity
- 46299
- Gene Name
- --
- Other Name
- POU domain class 5, transcription factor 1 (POU5F1), OCT4, OCT3
- Gene ID
- 5460
- Cellular Localization
- --
- Function
- Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Oct4 is a prime candidate for an early developmental control gene. It also is an important marker of the undifferentiated state and central regulator of pluripotency in ES cells.