- Target
- STAT1
- Reactivity
- Human, Mouse
- Applications
- WB,ICFC, ICC, ChIP,
- MW(Calculated)
- --
- Host Species
- Mouse
- Isotype
- Mouse IgG1, κ
- Recommended Dilution Ratio
- Each lot of this antibody is quality control tested by Western blotting . For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg/ml. For intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml is recommended. For ChIP applications, the suggested dilution is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Purification
- The antibody was purified by affinity chromatography.
- Storage
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Concentration
- 0.5 mg/ml
- Clonality
- Monoclonal
- Clone Number
- RMM5158
- Immunogen
- Human STAT1 peptide phosphorylated at Ser 727.
- Specificity
- STAT1 Phospho Ser727
- Gene Name
- --
- Other Name
- Signal transducer and activator of transcription 1 (STAT1), Transcription factor ISGF-3 components p91/p84
- Gene ID
- 6772
- Cellular Localization
- Nuclear Markers, Phospho-Proteins
- Function
- Phosphorylated in response to cytokine signaling by receptor-associated kinases; translocates to the nucleus to act as a transcription factor. Mediates responses to type I (IFN-α/β) and II interferon (IFN-γ), EGF, PDGF, and IL-6.