U-Blot® FoxO1A (Acetyl Lys245) rabbit pAb

Name: U-Blot® FoxO1A (Acetyl Lys245) rabbit pAb
SKU: WP0357
Availability: InStock

$288.00

Size:50μL

SKU: WP0357-50

Catalog No.: WP0357

Specification: 50μL/100μL

Stock: In stock

U-Blot® FoxO1A (Acetyl Lys245) rabbit pAb Learn more

Product details Specifications Q & A Education

Background：disease:Chromosomal aberrations involving FOXO1 are a cause of rhabdomyosarcoma 2 (RMS2) [MIM:268220]; also known as alveolar rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.,function:Transcription factor.,PTM:Phosphorylated by AKT1; insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 fork-head DNA-binding domain.,subcellular location:Shuttles between cytoplasm and nucleus.,subunit:Interacts with LRPPRC.,tissue specificity:Ubiquitous.,

Product Overview

Catalog No.: WP0357
SKU: WP0357-50
Category: Polyclonal antibodies
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: FoxO1
Reactivity: Human,Mouse,Rat
Application: WB,ELISA
MW(Calculated): --
MW(Observed): 72kD
Host Species: Rabbit
Isotype: IgG
Conjugate/Modification: Acetyl
Modification site: --
Recommended Dilution Ratio: WB 1:1000-2000;ELISA 1:5000-20000
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Polyclonal
Clone No.: --
Immunogen: Synthesized peptide derived from human FoxO1A (Acetyl Lys245)
Sequence: --
Specificity: This antibody detects endogenous levels of Human,Mouse,Rat FoxO1A (Acetyl Lys245)
Gene Name: FOXO1 FKHR FOXO1A
Protein Name: FoxO1A (Acetyl Lys245)
Other Name: Forkhead box protein O1;Forkhead box protein O1A;Forkhead in rhabdomyosarcoma;
Organism-1: Human
Gene ID-1: 2308
SwissProt-1: Q12778
Organism-2: Mouse
Gene ID-2: 56458
SwissProt-2: Q9R1E0
Organism-3: Rat
Gene ID-3: 84482
SwissProt-3: G3V7R4
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Cytoplasm . Nucleus . Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells (PubMed:11311120, PubMed:12228231, PubMed:19221179, PubMed:21245099, PubMed:20543840, PubMed:25009184). In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Serum deprivation increases localization to the nucleus, leading to activate expression of SOX9 and subsequent chondrogenesis (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway (PubMed:11237865, PubMed:12228231). Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation (PubMed:18356527). Phosphorylation by NLK results in nuclear export (By similarity). Translocates to the nucleus upon oxidative stress-induced phosphorylation at Ser-212 by STK4/MST1 (PubMed:19221179, PubMed:21245099). SGK1-mediated phosphorylation also results in nuclear translocation (By similarity). Retained in the nucleus under stress stimuli including oxidative stress, nutrient deprivation or nitric oxide (By similarity). Retained in the nucleus on methylation (By similarity). PPIA/CYPA stimulates its nuclear accumulation (PubMed:31063815). Deacetylation by SIRT6, promotes its translocation into the cytoplasm (PubMed:25009184). .