U-Blot® Vinculin (phospho Tyr821) Polyclonal Antibody

Name: U-Blot® Vinculin (phospho Tyr821) Polyclonal Antibody
SKU: WP10180
Availability: InStock

$288.00

Size:50μL

SKU: WP10180-50

Catalog No.: WP10180

Specification: 50μL/100μL

Stock: In stock

U-Blot® Vinculin (phospho Tyr821) Polyclonal Antibody Learn more

Product details Specifications Q & A Education

Background：Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008],

Product Overview

Catalog No.: WP10180
SKU: WP10180-50
Category: Polyclonal antibodies
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: Vinculin
Reactivity: Human,Mouse,Rat
Application: WB,IHC,IF,ELISA
MW(Calculated): 124kD
MW(Observed): --
Host Species: Rabbit
Isotype: IgG
Conjugate/Modification: Phospho
Modification site: --
Recommended Dilution Ratio: WB 1:500-2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Polyclonal
Clone No.: --
Immunogen: The antiserum was produced against synthesized peptide derived from human Vinculin around the phosphorylation site of Tyr821. AA range:786-835
Sequence: --
Specificity: Phospho-Vinculin (Y821) Polyclonal Antibody detects endogenous levels of Vinculin protein only when phosphorylated at Y821.
Gene Name: VCL
Protein Name: Vinculin
Other Name: VCL;Vinculin;Metavinculin
Organism-1: Human
Gene ID-1: 7414
SwissProt-1: P18206
Organism-2: Mouse
Gene ID-2: 22330
SwissProt-2: Q64727
Organism-3: Rat
Gene ID-3: 305679
SwissProt-3: P85972
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, adherens junction . Cell junction, focal adhesion . Cytoplasm, cytoskeleton . Cell membrane, sarcolemma ; Peripheral membrane protein ; Cytoplasmic side . Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions. .