U-Blot® MDM2 (phospho Ser186/S188) Polyclonal Antibody

Name: U-Blot® MDM2 (phospho Ser186/S188) Polyclonal Antibody
SKU: WP10221
Availability: InStock

$288.00

Size:50μL

SKU: WP10221-50

Catalog No.: WP10221

Specification: 50μL/100μL

Stock: In stock

U-Blot® MDM2 (phospho Ser186/S188) Polyclonal Antibody Learn more

Product details Specifications Q & A Education

Background：This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013],

Product Overview

Catalog No.: WP10221
SKU: WP10221-50
Category: Polyclonal antibodies
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: MDM2
Reactivity: Human,Rat,Mouse,
Application: IF,WB,ELISA
MW(Calculated): 55kD
MW(Observed): --
Host Species: Rabbit
Isotype: IgG
Conjugate/Modification: Phospho
Modification site: --
Recommended Dilution Ratio: WB 1:500-2000;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Polyclonal
Clone No.: --
Immunogen: The antiserum was produced against synthesized peptide derived from human MDM2 around the phosphorylation site of Ser186 and Ser188. AA range:151-200
Sequence: --
Specificity: Phospho-MDM2 (S186/S188) Polyclonal Antibody detects endogenous levels of MDM2 protein only when phosphorylated at S186/S188.
Gene Name: MDM2
Protein Name: E3 ubiquitin-protein ligase Mdm2
Other Name: MDM2;E3 ubiquitin-protein ligase Mdm2;Double minute 2 protein;Hdm2;Oncoprotein Mdm2;p53-binding protein Mdm2
Organism-1: Human
Gene ID-1: 4193
SwissProt-1: Q00987
Organism-2: Mouse
Gene ID-2: --
SwissProt-2: P23804
Organism-3: --
Gene ID-3: --
SwissProt-3: --
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Nucleus, nucleoplasm. Cytoplasm . Nucleus, nucleolus. Nucleus . Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.