U-Blot® ARF6 Polyclonal Antibody

Name: U-Blot® ARF6 Polyclonal Antibody
SKU: WP11111
Availability: InStock

$268.00

Size:50μL

SKU: WP11111-50

Catalog No.: WP11111

Specification: 50μL/100μL

Stock: In stock

U-Blot® ARF6 Polyclonal Antibody Learn more

Product details Specifications Q & A Education

Background：ADP ribosylation factor 6(ARF6) Homo sapiens This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq, Jul 2008],

Product Overview

Catalog No.: WP11111
SKU: WP11111-50
Category: Polyclonal antibodies
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: ARF6
Reactivity: Human,Mouse,Rat
Application: WB,ELISA
MW(Calculated): --
MW(Observed): 20kD
Host Species: Rabbit
Isotype: IgG
Conjugate/Modification: Unmodified
Modification site: --
Recommended Dilution Ratio: WB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications.
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Polyclonal
Clone No.: --
Immunogen: Synthesized peptide derived from ARF6 . at AA range: 30-110
Sequence: --
Specificity: ARF6 Polyclonal Antibody detects endogenous levels of ARF6 protein.
Gene Name: ARF6
Protein Name: ADP-ribosylation factor 6
Other Name: ARF6;ADP-ribosylation factor 6
Organism-1: Human
Gene ID-1: 382
SwissProt-1: P62330
Organism-2: Mouse
Gene ID-2: 11845
SwissProt-2: P62331
Organism-3: Rat
Gene ID-3: 79121
SwissProt-3: P62332
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Cytoplasm, cytosol . Cell membrane ; Lipid-anchor . Endosome membrane ; Lipid-anchor . Recycling endosome membrane ; Lipid-anchor . Cell projection, filopodium membrane ; Lipid-anchor . Cell projection, ruffle . Cleavage furrow . Midbody, Midbody ring . Early endosome membrane ; Lipid-anchor . Golgi apparatus, trans-Golgi network membrane ; Lipid-anchor . Distributed uniformly on the plasma membrane, as well as throughout the cytoplasm during metaphase. Subsequently concentrated at patches in the equatorial region at the onset of cytokinesis, and becomes distributed in the equatorial region concurrent with cleavage furrow ingression. In late stages of cytokinesis, concentrates at the midbody ring/Flemming body (PubMed:23603394). Recruitment to the midbody ring requires both activation by PSD/EFA6A and interaction with KIF23/MKLP1 (PubMed:23603394). After abscission of the intercellular bridge, incorporated into one of the daughter cells as a midbody remnant and localizes to punctate structures beneath the plasma membrane (PubMed:23603394). Recruited to the cell membrane in association with CYTH2 and ARL4C (PubMed:17398095). Colocalizes with DAB2IP at the plasma membrane and endocytic vesicles (PubMed:19948740). Myristoylation is required for proper localization to membranes: myristoylation on Lys-3 allows ARF6 to remain on membranes during the GTPase cycle (PubMed:7589240, PubMed:32103017). .