- Target
- HSP27
- Reactivity
- Human,Mouse,Rat
- Application
- IF,WB,IHC,ELISA
- MW(Calculated)
- --
- MW(Observed)
- 27kD
- Host Species
- Rabbit
- Isotype
- IgG
- Conjugate/Modification
- Unmodified
- Modification site
- --
- Recommended Dilution Ratio
- IF 1:50-200;WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:20000;Not yet tested in other applications;
- Form
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source
- --
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Purity
- --
- storageCondition
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Concentration
- 1 mg/ml
- Clonality
- Polyclonal
- Clone No.
- --
- Immunogen
- The antiserum was produced against synthesized peptide derived from human HSP27. AA range:48-97
- Sequence
- --
- Specificity
- HSP27 Polyclonal Antibody detects endogenous levels of HSP27 protein.
- Gene Name
- HSPB1
- Protein Name
- Heat shock protein beta-1
- Other Name
- HSPB1;HSP27;HSP28;Heat shock protein beta-1;HspB1;28 kDa heat shock protein;Estrogen-regulated 24 kDa protein;Heat shock 27 kDa protein;HSP 27;Stress-responsive protein 27;SRP27
- Organism-1
- Human
- Gene ID-1
- 3315
- SwissProt-1
- P04792
- Organism-2
- Mouse
- Gene ID-2
- 15507
- SwissProt-2
- P14602
- Organism-3
- Rat
- Gene ID-3
- 24471
- SwissProt-3
- P42930
- Organism-4
- --
- gene ID-4
- --
- SwissProt-4
- --
- Cellular Localization
- Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, spindle . Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles. .