- Target
- Melan-A
- Reactivity
- Human
- Application
- WB,IHC,IF,ELISA
- MW(Calculated)
- --
- MW(Observed)
- 15kD
- Host Species
- Rabbit
- Isotype
- IgG
- Conjugate/Modification
- Unmodified
- Modification site
- --
- Recommended Dilution Ratio
- WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200
- Form
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source
- --
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Purity
- --
- storageCondition
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Concentration
- 1 mg/ml
- Clonality
- Polyclonal
- Clone No.
- --
- Immunogen
- The antiserum was produced against synthesized peptide derived from human MART-1. AA range:41-90
- Sequence
- --
- Specificity
- Melan-A Polyclonal Antibody detects endogenous levels of Melan-A protein.
- Gene Name
- MLANA
- Protein Name
- Melanoma antigen recognized by T-cells 1
- Other Name
- MLANA;MART1;Melanoma antigen recognized by T-cells 1;MART-1;Antigen LB39-AA;Antigen SK29-AA;Protein Melan-A
- Organism-1
- Human
- Gene ID-1
- 2315
- SwissProt-1
- Q16655
- Organism-2
- --
- Gene ID-2
- --
- SwissProt-2
- --
- Organism-3
- --
- Gene ID-3
- --
- SwissProt-3
- --
- Organism-4
- --
- gene ID-4
- --
- SwissProt-4
- --
- Cellular Localization
- Endoplasmic reticulum membrane; Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.