U-Blot® MRTF-A Polyclonal Antibody

Name: U-Blot® MRTF-A Polyclonal Antibody
SKU: WP13294
Availability: InStock

$268.00

Size:50μL

SKU: WP13294-50

Catalog No.: WP13294

Specification: 50μL/100μL

Stock: In stock

U-Blot® MRTF-A Polyclonal Antibody Learn more

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Background：The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013],

Product Overview

Catalog No.: WP13294
SKU: WP13294-50
Category: Polyclonal antibodies
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: MRTF-A
Reactivity: Human,Mouse
Application: WB,IHC,IF,ELISA
MW(Calculated): --
MW(Observed): 99kD
Host Species: Rabbit
Isotype: IgG
Conjugate/Modification: Unmodified
Modification site: --
Recommended Dilution Ratio: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Polyclonal
Clone No.: --
Immunogen: The antiserum was produced against synthesized peptide derived from human MKL1. AA range:10-59
Sequence: --
Specificity: MRTF-A Polyclonal Antibody detects endogenous levels of MRTF-A protein.
Gene Name: MKL1
Protein Name: MKL/myocardin-like protein 1
Other Name: MKL1;KIAA1438;MAL;MKL/myocardin-like protein 1;Megakaryoblastic leukemia 1 protein;Megakaryocytic acute leukemia protein;Myocardin-related transcription factor A;MRTF-A
Organism-1: Human
Gene ID-1: 57591
SwissProt-1: Q969V6
Organism-2: Mouse
Gene ID-2: 223701
SwissProt-2: Q8K4J6
Organism-3: --
Gene ID-3: --
SwissProt-3: --
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Cytoplasm . Nucleus . Subcellular location is tightly regulated by actin both in cytoplasm and nucleus: high levels of G-actin in the nucleus observed during serum deprivation lead to low levels of nuclear MRTFA, while reduced levels of nuclear G-actin result in accumulation of MRTFA in the nucleus (By similarity). G-actin-binding in the cytoplasm inhibits nuclear import by masking the nuclear localization signal (NLS) (By similarity). In contrast, binding to nuclear globular actin (G-actin) promotes nuclear export to the cytoplasm (By similarity). Nuclear localization is regulated by MICAL2, which mediates depolymerization of nuclear actin, which decreases nuclear G-actin pool, thereby promoting retention of MRTFA in the nucleus and subsequent formation of an active complex with SRF (PubMed:24440334). .