U-Blot® Ref-1 Polyclonal Antibody

Name: U-Blot® Ref-1 Polyclonal Antibody
SKU: WP14242
Availability: InStock

$268.00

Size:50μL

SKU: WP14242-50

Catalog No.: WP14242

Specification: 50μL/100μL

Stock: In stock

U-Blot® Ref-1 Polyclonal Antibody Learn more

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Background：Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq, Jul 2008],

Product Overview

Catalog No.: WP14242
SKU: WP14242-50
Category: Polyclonal antibodies
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: Ref-1
Reactivity: Human,Mouse,Rat
Application: WB,IHC,IF,ELISA
MW(Calculated): --
MW(Observed): 34kD
Host Species: Rabbit
Isotype: IgG
Conjugate/Modification: Unmodified
Modification site: --
Recommended Dilution Ratio: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:20000;IF 1:50-200
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Polyclonal
Clone No.: --
Immunogen: The antiserum was produced against synthesized peptide derived from human APEX1. AA range:191-240
Sequence: --
Specificity: Ref-1 Polyclonal Antibody detects endogenous levels of Ref-1 protein.
Gene Name: APEX1
Protein Name: DNA-(apurinic or apyrimidinic site) lyase
Other Name: APEX1;APE;APE1;APEX;APX;HAP1;REF1;DNA-;apurinic or apyrimidinic site;lyase;APEX nuclease;APEN;Apurinic-apyrimidinic endonuclease 1;AP endonuclease 1;APE-1;REF-1;Redox factor-1
Organism-1: Human
Gene ID-1: 328
SwissProt-1: P27695
Organism-2: Mouse
Gene ID-2: 11792
SwissProt-2: P28352
Organism-3: Rat
Gene ID-3: 79116
SwissProt-3: P43138
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Nucleus. Nucleus, nucleolus. Nucleus speckle. Endoplasmic reticulum. Cytoplasm. Detected in the cytoplasm of B-cells stimulated to switch (By similarity). Colocalized with SIRT1 in the nucleus. Colocalized with YBX1 in nuclear speckles after genotoxic stress. Together with OGG1 is recruited to nuclear speckles in UVA-irradiated cells. Colocalized with nucleolin and NPM1 in the nucleolus. Its nucleolar localization is cell cycle dependent and requires active rRNA transcription. Colocalized with calreticulin in the endoplasmic reticulum. Translocation from the nucleus to the cytoplasm is stimulated in presence of nitric oxide (NO) and function in a CRM1-dependent manner, possibly as a consequence of demasking a nuclear export signal (amino acid position 64-80). S-nitrosylation at Cys-93 and Cys-310 regulates its nuclear-cytosolic shuttling. Ubiquitinated form is localized predominantly in the cytoplasm. .; [DNA-(apurinic or apyrimidinic site) endonuclease, mitochondrial]: Mitochondrion. The cleaved APEX2 is only detected in mitochondria (By similarity). Translocation from the cytoplasm to the mitochondria is mediated by ROS signaling and cleavage mediated by granzyme A. Tom20-dependent translocated mitochondrial APEX1 level is significantly increased after genotoxic stress. .