- Target
- RFWD3
- Reactivity
- Human,Mouse
- Application
- WB,IHC,IF,ELISA
- MW(Calculated)
- --
- MW(Observed)
- 85kD
- Host Species
- Rabbit
- Isotype
- IgG
- Conjugate/Modification
- Unmodified
- Modification site
- --
- Recommended Dilution Ratio
- WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200
- Form
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source
- --
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Purity
- --
- storageCondition
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Concentration
- 1 mg/ml
- Clonality
- Polyclonal
- Clone No.
- --
- Immunogen
- The antiserum was produced against synthesized peptide derived from human RFWD3. AA range:374-423
- Sequence
- --
- Specificity
- RFWD3 Polyclonal Antibody detects endogenous levels of RFWD3 protein.
- Gene Name
- RFWD3
- Protein Name
- E3 ubiquitin-protein ligase RFWD3
- Other Name
- RFWD3;RNF201;E3 ubiquitin-protein ligase RFWD3;RING finger and WD repeat domain-containing protein 3;RING finger protein 201
- Organism-1
- Human
- Gene ID-1
- 55159
- SwissProt-1
- Q6PCD5
- Organism-2
- Mouse
- Gene ID-2
- --
- SwissProt-2
- Q8CIK8
- Organism-3
- --
- Gene ID-3
- --
- SwissProt-3
- --
- Organism-4
- --
- gene ID-4
- --
- SwissProt-4
- --
- Cellular Localization
- Nucleus . Nucleus, PML body . Cytoplasm . In undamaged cells, found both in the cytoplasm and in the nucleus, partially associated with PML nuclear bodies (PubMed:21558276). In response to replication block, such as that caused by hydroxyurea treatment, or to DNA damage caused by ionizing radiations or doxorubicin, recruited to the nucleus, to stalled replication forks or to sites of DNA repair (PubMed:21504906, PubMed:28575657). This recruitment depends upon RPA2 (PubMed:21504906). .