U-Blot® HMG-1 Polyclonal Antibody

Name: U-Blot® HMG-1 Polyclonal Antibody
SKU: WP15523
Availability: InStock

$268.00

Size:50μL

SKU: WP15523-50

Catalog No.: WP15523

Specification: 50μL/100μL

Stock: In stock

U-Blot® HMG-1 Polyclonal Antibody Learn more

Product details Specifications Q & A Education

Background：This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015],

Product Overview

Catalog No.: WP15523
SKU: WP15523-50
Category: Polyclonal antibodies
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: HMGB1
Reactivity: Human,Mouse,Rat
Application: IF,WB,IHC,ELISA
MW(Calculated): --
MW(Observed): 30kD
Host Species: Rabbit
Isotype: IgG
Conjugate/Modification: Unmodified
Modification site: --
Recommended Dilution Ratio: IF 1:50-200;WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:20000;Not yet tested in other applications;
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Polyclonal
Clone No.: --
Immunogen: Synthesized peptide derived from the N-terminal region of human HMG-1.
Sequence: --
Specificity: HMG-1 Polyclonal Antibody detects endogenous levels of HMG-1 protein.
Gene Name: HMGB1
Protein Name: High mobility group protein B1
Other Name: HMGB1;HMG1;High mobility group protein B1;High mobility group protein 1;HMG-1
Organism-1: Human
Gene ID-1: 3146
SwissProt-1: P09429
Organism-2: Mouse
Gene ID-2: 100862258
SwissProt-2: P63158
Organism-3: Rat
Gene ID-3: 25459
SwissProt-3: P63159
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Nucleus . Chromosome . Cytoplasm . Secreted . Cell membrane ; Peripheral membrane protein ; Extracellular side . Endosome . Endoplasmic reticulum-Golgi intermediate compartment . In basal state predominantly nuclear. Shuttles between the cytoplasm and the nucleus (PubMed:12231511, PubMed:17114460). Translocates from the nucleus to the cytoplasm upon autophagy stimulation (PubMed:20819940). Release from macrophages in the extracellular milieu requires the activation of NLRC4 or NLRP3 inflammasomes (By similarity). Passively released to the extracellular milieu from necrotic cells by diffusion, involving the fully reduced HGMB1 which subsequently gets oxidized (PubMed:19811284). Also released from apoptotic cells (PubMed:16855214, PubMed:18631454). Active secretion from a variety of immune and non-immune cells such as macrophages, monocytes, neutrophils, dendritic cells and natural killer cells in response to various stimuli such as LPS and cytokines involves a nonconventional secretory process via secretory lysosomes (PubMed:12231511, PubMed:14532127, PubMed:15944249). Secreted by plasma cells in response to LPS (By similarity). Found on the surface of activated platelets (PubMed:11154118). An increased chromatin association is observed when associated with the adenovirus protein pVII (PubMed:27362237). .