- Target
- HSP A5/GRP78
- Reactivity
- Human,Mouse,Rat,Fish
- Application
- WB,IHC,IF,ELISA
- MW(Calculated)
- --
- MW(Observed)
- 78kD
- Host Species
- Rabbit
- Isotype
- IgG
- Conjugate/Modification
- Unmodified
- Modification site
- --
- Recommended Dilution Ratio
- WB 1:500-1:2000;IHC-f:1:50-300;ELISA 1:10000-20000;IF 1:50-200
- Form
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source
- --
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Purity
- --
- storageCondition
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Concentration
- 1 mg/ml
- Clonality
- Polyclonal
- Clone No.
- --
- Immunogen
- Synthetic peptide from human protein at AA range: 505-570
- Sequence
- --
- Specificity
- The antibody detects endogenous GRP78 BiP
- Gene Name
- HSPA5 GRP78
- Protein Name
- GRP78 BiP
- Other Name
- 78 kDa glucose-regulated protein;GRP-78;Endoplasmic reticulum lumenal Ca;2+;-binding protein grp78;Heat shock 70 kDa protein 5;Immunoglobulin heavy chain-binding protein;BiP;
- Organism-1
- Human
- Gene ID-1
- 3309
- SwissProt-1
- P11021
- Organism-2
- Mouse
- Gene ID-2
- 14828
- SwissProt-2
- P20029
- Organism-3
- Rat
- Gene ID-3
- --
- SwissProt-3
- P06761
- Organism-4
- --
- gene ID-4
- --
- SwissProt-4
- --
- Cellular Localization
- Endoplasmic reticulum lumen . Melanosome . Cytoplasm . Cell surface . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545). Localizes to the cell surface of epithelial cells in response to high levels of free iron (PubMed:20484814, PubMed:24355926, PubMed:27159390). .