- Target
- COPB
- Reactivity
- Human,Rat,Mouse,
- Application
- WB,ELISA
- MW(Calculated)
- --
- MW(Observed)
- 104kD
- Host Species
- Rabbit
- Isotype
- IgG
- Conjugate/Modification
- Unmodified
- Modification site
- --
- Recommended Dilution Ratio
- WB 1:500-2000;ELISA 1:5000-20000
- Form
- PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
- Source
- --
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Purity
- --
- storageCondition
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Concentration
- 1 mg/ml
- Clonality
- Polyclonal
- Clone No.
- --
- Immunogen
- Synthesized peptide derived from part region of human protein AA range: 865-915
- Sequence
- --
- Specificity
- COPB Polyclonal Antibody detects endogenous levels of protein.
- Gene Name
- COPB1 COPB MSTP026
- Protein Name
- Coatomer subunit beta (Beta-coat protein) (Beta-COP)
- Other Name
- --
- Organism-1
- Human
- Gene ID-1
- 1315
- SwissProt-1
- P53618
- Organism-2
- Mouse
- Gene ID-2
- --
- SwissProt-2
- Q9JIF7
- Organism-3
- Rat
- Gene ID-3
- --
- SwissProt-3
- P23514
- Organism-4
- --
- gene ID-4
- --
- SwissProt-4
- --
- Cellular Localization
- Cytoplasm. Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasmic vesicle, COPI-coated vesicle membrane; Peripheral membrane protein ; Cytoplasmic side . Cell membrane . Endoplasmic reticulum-Golgi intermediate compartment . The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it (By similarity). Proteolytic cleavage by CAPN8 triggers translocation from Golgi to cytoplasm (By similarity). Found in perinuclear vesicular-tubular clusters (VTCs) and in the Golgi region where associated with vesicles, buds and rims of the Golgi stack (By similarity). Occasionally present at the trans-side of Golgi, but mainly present at the cis-Golgi side in transitional areas (TA), on so-called peripheral elements (PE) consisting of tubules and vesicles located between the cup-shaped transitional elements (TE) of the rough endoplasmic reticulum (RER) and the cis-most Golgi cisternae (By similarity). Present in cytoplasm, not associated with visible coats or membranes, with a minor fraction present on small clusters of tubules and vesicles (By similarity). Some association with high-density and low-density microsomes and mitochondria/nuclei fraction (By similarity). Very little found in plasma membrane fraction (PubMed:20362547). .