- Target
- SKAP
- Reactivity
- Human,Mouse,Rat
- Application
- WB
- MW(Calculated)
- 35kD
- MW(Observed)
- --
- Host Species
- Rabbit
- Isotype
- IgG
- Conjugate/Modification
- Unmodified
- Modification site
- --
- Recommended Dilution Ratio
- WB 1:1000-2000
- Form
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source
- --
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Purity
- --
- storageCondition
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Concentration
- 1 mg/ml
- Clonality
- Polyclonal
- Clone No.
- --
- Immunogen
- Synthesized peptide derived from human SKAP AA range: 34-84
- Sequence
- --
- Specificity
- This antibody detects endogenous levels of human SKAP
- Gene Name
- SKAP C15orf23 TRAF4AF1 HSD11
- Protein Name
- Small kinetochore-associated protein (SKAP) (Kinetochore-localized astrin-binding protein) (Kinastrin) (TRAF4-associated factor 1)
- Other Name
- --
- Organism-1
- Human
- Gene ID-1
- 90417
- SwissProt-1
- Q9Y448
- Organism-2
- Mouse
- Gene ID-2
- 51944
- SwissProt-2
- Q9D9Z1
- Organism-3
- Rat
- Gene ID-3
- 51944
- SwissProt-3
- Q6AXN6
- Organism-4
- --
- gene ID-4
- --
- SwissProt-4
- --
- Cellular Localization
- Nucleus . Chromosome, centromere, kinetochore . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cytoskeleton, microtubule organizing center . Colocalizes with microtubules around centrosomes in prophase and with the mitotic spindle at prometaphase and metaphase. From late prometaphase to anaphase, is highly concentrated on kinetochores. Located at the kinetochore-microtubule interface. The astrin (SPAG5)-kinastrin (SKAP) complex localizes to the microtubule plus ends (PubMed:23035123). .