- Target
- GBF1
- Reactivity
- Human
- Application
- WB
- MW(Calculated)
- 204kD
- MW(Observed)
- --
- Host Species
- Rabbit
- Isotype
- IgG
- Conjugate/Modification
- Unmodified
- Modification site
- --
- Recommended Dilution Ratio
- WB 1:500-2000
- Form
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source
- --
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Purity
- --
- storageCondition
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Concentration
- 1 mg/ml
- Clonality
- Polyclonal
- Clone No.
- --
- Immunogen
- Synthesized peptide derived from human GBF1
- Sequence
- --
- Specificity
- This antibody detects endogenous levels of GBF1 at Human
- Gene Name
- GBF1 KIAA0248
- Protein Name
- Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 (BFA-resistant GEF 1)
- Other Name
- --
- Organism-1
- Human
- Gene ID-1
- 8729
- SwissProt-1
- Q92538
- Organism-2
- --
- Gene ID-2
- --
- SwissProt-2
- --
- Organism-3
- --
- Gene ID-3
- --
- SwissProt-3
- --
- Organism-4
- --
- gene ID-4
- --
- SwissProt-4
- --
- Cellular Localization
- Golgi apparatus, cis-Golgi network . Endoplasmic reticulum-Golgi intermediate compartment . Golgi apparatus, trans-Golgi network . Golgi apparatus . Cytoplasm . Lipid droplet . Membrane; Peripheral membrane protein . Cycles rapidly on and off early Golgi membranes (PubMed:15616190). Stabilized on membranes when complexed with ARF1-GDP and is released from both ARF1 and membranes after it catalyzes GDP displacement and ARF1 binds GTP. Continuous cycles of recruitment and dissociation of GBF1 to membranes are required for sustained ARF activation and COP I recruitment (PubMed:15813748). In neutrophils is translocated from the Golgi to the leading edge upon GPCR stimulation (PubMed:22573891). Localization to lipid droplets is questionable (PubMed:22185782). .