U-Blot® PDPK1 (phospho Ser241) Polyclonal Antibody

Name: U-Blot® PDPK1 (phospho Ser241) Polyclonal Antibody
SKU: WP9255
Availability: InStock

$288.00

Size:50μL

SKU: WP9255-50

Catalog No.: WP9255

Specification: 50μL/100μL

Stock: In stock

U-Blot® PDPK1 (phospho Ser241) Polyclonal Antibody Learn more

Product details Specifications Q & A Education

Background：catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.,PTM:Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.,subunit:Interacts with TUSC4.,tissue specificity:Appears to be expressed ubiquitously.,

Product Overview

Catalog No.: WP9255
SKU: WP9255-50
Category: Polyclonal antibodies
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: PDPK1
Reactivity: Human,Mouse,Rat
Application: WB,IHC,IF,ELISA
MW(Calculated): --
MW(Observed): 63kD
Host Species: Rabbit
Isotype: IgG
Conjugate/Modification: Phospho
Modification site: --
Recommended Dilution Ratio: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Polyclonal
Clone No.: --
Immunogen: The antiserum was produced against synthesized peptide derived from human PDK1 around the phosphorylation site of Ser241. AA range:210-259
Sequence: --
Specificity: Phospho-PDK1 (S241) Polyclonal Antibody detects endogenous levels of PDK1 protein only when phosphorylated at S241.
Gene Name: PDPK1
Protein Name: 3-phosphoinositide-dependent protein kinase 1
Other Name: PDPK1;PDK1;3-phosphoinositide-dependent protein kinase 1;hPDK1
Organism-1: Human
Gene ID-1: 5170
SwissProt-1: O15530
Organism-2: Mouse
Gene ID-2: 18607
SwissProt-2: Q9Z2A0
Organism-3: Rat
Gene ID-3: 81745
SwissProt-3: O55173
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Cell junction, focal adhesion. Tyrosine phosphorylation seems to occur only at the cell membrane. Translocates to the cell membrane following insulin stimulation by a mechanism that involves binding to GRB14 and INSR. SRC and HSP90 promote its localization to the cell membrane. Its nuclear localization is dependent on its association with PTPN6 and its phosphorylation at Ser-396. Restricted to the nucleus in neuronal cells while in non-neuronal cells it is found in the cytoplasm. The Ser-241 phosphorylated form is distributed along the perinuclear region in neuronal cells while in non-neuronal cells it is found in both the nucleus and the cytoplasm. IGF1 transiently increases phosphorylation at Ser-241 of neuronal PDPK1, resulting in its translocation to other cellular compartments. The tyrosine-phosphorylated form colocalizes with PTK2B in focal adhesions after angiotensin II stimulation.