U-Blot® FoxO1/3 (phospho Ser322/S325) Polyclonal Antibody

Name: U-Blot® FoxO1/3 (phospho Ser322/S325) Polyclonal Antibody
SKU: WP9338
Availability: InStock

$288.00

Size:50μL

SKU: WP9338-50

Catalog No.: WP9338

Specification: 50μL/100μL

Stock: In stock

U-Blot® FoxO1/3 (phospho Ser322/S325) Polyclonal Antibody Learn more

Product details Specifications Q & A Education

Background：This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008],

Product Overview

Catalog No.: WP9338
SKU: WP9338-50
Category: Polyclonal antibodies
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: FoxO1/3
Reactivity: Human,Mouse,Rat
Application: WB,IHC,IF,ELISA
MW(Calculated): --
MW(Observed): 97kD
Host Species: Rabbit
Isotype: IgG
Conjugate/Modification: Phospho
Modification site: --
Recommended Dilution Ratio: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Polyclonal
Clone No.: --
Immunogen: The antiserum was produced against synthesized peptide derived from human FOXO1A/3A around the phosphorylation site of Ser322 and Ser325. AA range:291-340
Sequence: --
Specificity: Phospho-FoxO1/3 (S322/S325) Polyclonal Antibody detects endogenous levels of FoxO1/3 protein only when phosphorylated at S322/S325.
Gene Name: FOXO1/FOXO3
Protein Name: Forkhead box protein O1/3
Other Name: FOXO1;FKHR;FOXO1A;Forkhead box protein O1;Forkhead box protein O1A;Forkhead in rhabdomyosarcoma;FOXO3;FKHRL1;FOXO3A;Forkhead box protein O3;AF6q21 protein;Forkhead in rhabdomyosarcoma-like 1
Organism-1: Human
Gene ID-1: 2308
SwissProt-1: Q12778;O43524
Organism-2: Mouse
Gene ID-2: 56458;56484
SwissProt-2: --
Organism-3: Rat
Gene ID-3: 84482
SwissProt-3: G3V7R4
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Cytoplasm . Nucleus . Shuttles between the cytoplasm and nucleus. Largely nuclear in unstimulated cells (PubMed:11311120, PubMed:12228231, PubMed:19221179, PubMed:21245099, PubMed:20543840, PubMed:25009184). In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity). Serum deprivation increases localization to the nucleus, leading to activate expression of SOX9 and subsequent chondrogenesis (By similarity). Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway (PubMed:11237865, PubMed:12228231). Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation (PubMed:18356527). Phosphorylation by NLK results in nuclear export (By similarity). Translocates to the nucleus upon oxidative stress-induced phosphorylation at Ser-212 by STK4/MST1 (PubMed:19221179, PubMed:21245099). SGK1-mediated phosphorylation also results in nuclear translocation (By similarity). Retained in the nucleus under stress stimuli including oxidative stress, nutrient deprivation or nitric oxide (By similarity). Retained in the nucleus on methylation (By similarity). PPIA/CYPA stimulates its nuclear accumulation (PubMed:31063815). Deacetylation by SIRT6, promotes its translocation into the cytoplasm (PubMed:25009184). .