U-Blot® Smad2/3 (phospho Thr8) Polyclonal Antibody

Name: U-Blot® Smad2/3 (phospho Thr8) Polyclonal Antibody
SKU: WP9395
Availability: InStock

$288.00

Size:50μL

SKU: WP9395-50

Catalog No.: WP9395

Specification: 50μL/100μL

Stock: In stock

U-Blot® Smad2/3 (phospho Thr8) Polyclonal Antibody Learn more

Product details Specifications Q & A Education

Background：The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation

Product Overview

Catalog No.: WP9395
SKU: WP9395-50
Category: Polyclonal antibodies
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: Smad2/3
Reactivity: Human,Mouse,Rat,Pig
Application: WB,IF,ELISA
MW(Calculated): --
MW(Observed): 48kD
Host Species: Rabbit
Isotype: IgG
Conjugate/Modification: Phospho
Modification site: --
Recommended Dilution Ratio: WB 1:500-2000;IF/ICC1:100-500;ELISA 1:5000-20000
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Polyclonal
Clone No.: --
Immunogen: The antiserum was produced against synthesized peptide derived from human Smad2/3 around the phosphorylation site of Thr8. AA range:1-50
Sequence: --
Specificity: Phospho-Smad2/3 (T8) Polyclonal Antibody detects endogenous levels of Smad2/3 protein only when phosphorylated at T8.
Gene Name: SMAD2/SMAD3
Protein Name: Mothers against decapentaplegic homolog 2/3
Other Name: SMAD2;MADH2;MADR2;Mothers against decapentaplegic homolog 2;MAD homolog 2;Mothers against DPP homolog 2;JV18-1;Mad-related protein 2;hMAD-2;SMAD family member 2;SMAD 2;Smad2;hSMAD2;SMAD3;MADH3;Mothers against decapentaplegic
Organism-1: Human
Gene ID-1: 4087;4088
SwissProt-1: Q15796;P84022
Organism-2: Mouse
Gene ID-2: 17126;17127
SwissProt-2: --
Organism-3: Rat
Gene ID-3: 29357;25631
SwissProt-3: O70436;P84025
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Cytoplasm . Nucleus . Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:9865696, PubMed:21145499). On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm at the blastocyst and epiblast stages (By similarity). .