U-Blot® Plk1 (phospho Ser137) Polyclonal Antibody

Name: U-Blot® Plk1 (phospho Ser137) Polyclonal Antibody
SKU: WP9467
Availability: InStock

$288.00

Size:50μL

SKU: WP9467-50

Catalog No.: WP9467

Specification: 50μL/100μL

Stock: In stock

U-Blot® Plk1 (phospho Ser137) Polyclonal Antibody Learn more

Product details Specifications Q & A Education

Background：The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015],

Product Overview

Catalog No.: WP9467
SKU: WP9467-50
Category: Polyclonal antibodies
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: PLK1
Reactivity: Human,Mouse,Rat
Application: WB,IF,ELISA,IHC
MW(Calculated): --
MW(Observed): 68kD
Host Species: Rabbit
Isotype: IgG
Conjugate/Modification: Phospho
Modification site: --
Recommended Dilution Ratio: WB 1:500-2000;IF/ICC1:50-200;ELISA 1:2000-20000;IHC 1:50-200
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Polyclonal
Clone No.: --
Immunogen: The antiserum was produced against synthesized peptide derived from human PLK1 around the phosphorylation site of Ser137. AA range:103-152
Sequence: --
Specificity: Phospho-Plk1 (S137) Polyclonal Antibody detects endogenous levels of Plk1 protein only when phosphorylated at S137.
Gene Name: PLK1
Protein Name: Serine/threonine-protein kinase PLK1
Other Name: PLK1;PLK;Serine/threonine-protein kinase PLK1;Polo-like kinase 1;PLK-1;Serine/threonine-protein kinase 13;STPK13
Organism-1: Human
Gene ID-1: 5347
SwissProt-1: P53350
Organism-2: Mouse
Gene ID-2: 18817
SwissProt-2: Q07832
Organism-3: Rat
Gene ID-3: 25515
SwissProt-3: Q62673
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Midbody . localization at the centrosome starts at the G1/S transition (PubMed:24018379). During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGO1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization. Localizes onto the central spindle by phosphorylating and docking at midzone proteins KIF20A/MKLP2 and PRC1. Colocalizes with FRY to separating centrosomes and spindle poles from prophase to metaphase in mitosis, but not in other stages of the cell cycle. Localization to the centrosome is required for S phase progression (PubMed:24018379). Colocalizes with HSF1 at the spindle poles during prometaphase (PubMed:18794143). .