U-Blot® Shc (phospho Tyr427) Polyclonal Antibody

Name: U-Blot® Shc (phospho Tyr427) Polyclonal Antibody
SKU: WP9611
Availability: InStock

$288.00

Size:50μL

SKU: WP9611-50

Catalog No.: WP9611

Specification: 50μL/100μL

Stock: In stock

U-Blot® Shc (phospho Tyr427) Polyclonal Antibody Learn more

Product details Specifications Q & A Education

Background：This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011],

Product Overview

Catalog No.: WP9611
SKU: WP9611-50
Category: Polyclonal antibodies
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: Shc
Reactivity: Human,Mouse,Rat
Application: WB,IHC,IF,ELISA
MW(Calculated): --
MW(Observed): 66(p66 isoform),52(p52 isoform),46(p46 isoform)kD
Host Species: Rabbit
Isotype: IgG
Conjugate/Modification: Phospho
Modification site: --
Recommended Dilution Ratio: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Polyclonal
Clone No.: --
Immunogen: The antiserum was produced against synthesized peptide derived from human Shc around the phosphorylation site of Tyr427. AA range:393-442
Sequence: --
Specificity: Phospho-Shc (Y427) Polyclonal Antibody detects endogenous levels of Shc protein only when phosphorylated at Y427.
Gene Name: SHC1
Protein Name: SHC-transforming protein 1
Other Name: SHC1;SHC;SHCA;SHC-transforming protein 1;SHC-transforming protein 3;SHC-transforming protein A;Src homology 2 domain-containing-transforming protein C1;SH2 domain protein C1
Organism-1: Human
Gene ID-1: 6464
SwissProt-1: P29353
Organism-2: Mouse
Gene ID-2: 20416
SwissProt-2: P98083
Organism-3: Rat
Gene ID-3: 85385
SwissProt-3: Q5M824
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Cytoplasm.; [Isoform p46Shc]: Mitochondrion matrix . Localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52Shc and isoform p66Shc, that contain the same sequence but more internally located, display a different subcellular localization.; [Isoform p66Shc]: Mitochondrion . In case of oxidative conditions, phosphorylation at 'Ser-36' of isoform p66Shc, leads to mitochondrial accumulation. .