U-Blot® SMC1 (phospho Ser966) Polyclonal Antibody

Name: U-Blot® SMC1 (phospho Ser966) Polyclonal Antibody
SKU: WP9703
Availability: InStock

$288.00

Size:50μL

SKU: WP9703-50

Catalog No.: WP9703

Specification: 50μL/100μL

Stock: In stock

U-Blot® SMC1 (phospho Ser966) Polyclonal Antibody Learn more

Product details Specifications Q & A Education

Background：structural maintenance of chromosomes 1A(SMC1A) Homo sapiens Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Altern

Product Overview

Catalog No.: WP9703
SKU: WP9703-50
Category: Polyclonal antibodies
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: SMC1
Reactivity: Human,Mouse,Rat
Application: WB,IHC,IF,ELISA
MW(Calculated): --
MW(Observed): 160kD
Host Species: Rabbit
Isotype: IgG
Conjugate/Modification: Phospho
Modification site: --
Recommended Dilution Ratio: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Polyclonal
Clone No.: --
Immunogen: The antiserum was produced against synthesized peptide derived from human SMC1 around the phosphorylation site of Ser966. AA range:932-981
Sequence: --
Specificity: Phospho-SMC1 (S966) Polyclonal Antibody detects endogenous levels of SMC1 protein only when phosphorylated at S966.
Gene Name: SMC1A
Protein Name: Structural maintenance of chromosomes protein 1A
Other Name: SMC1A;DXS423E;KIAA0178;SB1.8;SMC1;SMC1L1;Structural maintenance of chromosomes protein 1A;SMC protein 1A;SMC-1-alpha;SMC-1A;Sb1.8
Organism-1: Human
Gene ID-1: 8243
SwissProt-1: Q14683
Organism-2: Mouse
Gene ID-2: 24061
SwissProt-2: Q9CU62
Organism-3: Rat
Gene ID-3: 63996
SwissProt-3: Q9Z1M9
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Nucleus . Chromosome . Chromosome, centromere, kinetochore . Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis.