U-Blot® 5-LO (phospho Ser272) Polyclonal Antibody

Name: U-Blot® 5-LO (phospho Ser272) Polyclonal Antibody
SKU: WP9747
Availability: InStock

$288.00

Size:50μL

SKU: WP9747-50

Catalog No.: WP9747

Specification: 50μL/100μL

Stock: In stock

U-Blot® 5-LO (phospho Ser272) Polyclonal Antibody Learn more

Product details Specifications Q & A Education

Background：This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012],

Product Overview

Catalog No.: WP9747
SKU: WP9747-50
Category: Polyclonal antibodies
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: 5-LO
Reactivity: Human,Mouse,Rat
Application: WB,IHC,IF,ELISA
MW(Calculated): --
MW(Observed): 78kD
Host Species: Rabbit
Isotype: IgG
Conjugate/Modification: Phospho
Modification site: --
Recommended Dilution Ratio: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Polyclonal
Clone No.: --
Immunogen: The antiserum was produced against synthesized peptide derived from human Arachidonate 5 Lipoxygenase around the phosphorylation site of Ser271. AA range:246-295
Sequence: --
Specificity: Phospho-5-LO (S272) Polyclonal Antibody detects endogenous levels of 5-LO protein only when phosphorylated at S272.
Gene Name: ALOX5
Protein Name: Arachidonate 5-lipoxygenase
Other Name: ALOX5;LOG5;Arachidonate 5-lipoxygenase;5-LO;5-lipoxygenase
Organism-1: Human
Gene ID-1: 240
SwissProt-1: P09917
Organism-2: Mouse
Gene ID-2: 11689
SwissProt-2: P48999
Organism-3: Rat
Gene ID-3: 25290
SwissProt-3: P12527
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Cytoplasm . Nucleus matrix . Nucleus membrane ; Peripheral membrane protein . Cytoplasm, perinuclear region . Cytoplasm, cytosol . Nucleus envelope . Nucleus intermembrane space . Shuttles between cytoplasm and nucleus (PubMed:19233132). Found exclusively in the nucleus, when phosphorylated on Ser-272 (PubMed:18978352). Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association (PubMed:19233132, PubMed:3118366, PubMed:8245774, PubMed:16275640). .