U-Blot® PRAK (phospho Thr182) Polyclonal Antibody

Name: U-Blot® PRAK (phospho Thr182) Polyclonal Antibody
SKU: WP9852
Availability: InStock

$288.00

Size:50μL

SKU: WP9852-50

Catalog No.: WP9852

Specification: 50μL/100μL

Stock: In stock

U-Blot® PRAK (phospho Thr182) Polyclonal Antibody Learn more

Product details Specifications Q & A Education

Background：The protein encoded by this gene is a tumor suppressor and member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. The encoded protein is found in the nucleus but translocates to the cytoplasm upon phosphorylation and activation. This kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2012],

Product Overview

Catalog No.: WP9852
SKU: WP9852-50
Category: Polyclonal antibodies
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: MAPKAPK5
Reactivity: Human,Mouse
Application: WB,IHC,IF,ELISA
MW(Calculated): --
MW(Observed): 60kD
Host Species: Rabbit
Isotype: IgG
Conjugate/Modification: Phospho
Modification site: --
Recommended Dilution Ratio: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Polyclonal
Clone No.: --
Immunogen: The antiserum was produced against synthesized peptide derived from human MAPKAPK5 around the phosphorylation site of Thr182. AA range:148-197
Sequence: --
Specificity: Phospho-PRAK (T182) Polyclonal Antibody detects endogenous levels of PRAK protein only when phosphorylated at T182.
Gene Name: MAPKAPK5
Protein Name: MAP kinase-activated protein kinase 5
Other Name: MAPKAPK5;PRAK;MAP kinase-activated protein kinase 5;MAPK-activated protein kinase 5;MAPKAP kinase 5;MAPKAP-K5;MAPKAPK-5;MK-5;MK5;p38-regulated/activated protein kinase;PRAK
Organism-1: Human
Gene ID-1: 8550
SwissProt-1: Q8IW41
Organism-2: Mouse
Gene ID-2: 17165
SwissProt-2: O54992
Organism-3: --
Gene ID-3: --
SwissProt-3: --
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Cytoplasm. Nucleus. Translocates to the cytoplasm following phosphorylation and activation. Interaction with ERK3/MAPK6 or ERK4/MAPK4 and phosphorylation at Thr-182, activates the protein kinase activity, followed by translocation to the cytoplasm. Phosphorylation by PKA/PRKACA at Ser-115 also induces nuclear export.