U-Blot® NFκB-p65 (phospho Ser311) Polyclonal Antibody

Name: U-Blot® NFκB-p65 (phospho Ser311) Polyclonal Antibody
SKU: WP9879
Availability: InStock

$288.00

Size:50μL

SKU: WP9879-50

Catalog No.: WP9879

Specification: 50μL/100μL

Stock: In stock

U-Blot® NFκB-p65 (phospho Ser311) Polyclonal Antibody Learn more

Product details Specifications Q & A Education

Background：NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],

Product Overview

Catalog No.: WP9879
SKU: WP9879-50
Category: Polyclonal antibodies
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: NFkB p65
Reactivity: Human,Mouse
Application: WB,IHC,IF,ELISA
MW(Calculated): --
MW(Observed): 65kD
Host Species: Rabbit
Isotype: IgG
Conjugate/Modification: Phospho
Modification site: --
Recommended Dilution Ratio: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:40000;Not yet tested in other applications.
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Polyclonal
Clone No.: --
Immunogen: The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Ser311. AA range:278-327
Sequence: --
Specificity: Phospho-NFκB-p65 (S311) Polyclonal Antibody detects endogenous levels of NFκB-p65 protein only when phosphorylated at S311.
Gene Name: RELA
Protein Name: Transcription factor p65
Other Name: RELA;NFKB3;Transcription factor p65;Nuclear factor NF-kappa-B p65 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Organism-1: Human
Gene ID-1: 5970
SwissProt-1: Q04206
Organism-2: Mouse
Gene ID-2: 19697
SwissProt-2: Q04207
Organism-3: --
Gene ID-3: --
SwissProt-3: --
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Nucleus . Cytoplasm . Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B) (PubMed:1493333). Colocalized with DDX1 in the nucleus upon TNF-alpha induction (PubMed:19058135). Colocalizes with GFI1 in the nucleus after LPS stimulation (PubMed:20547752). Translocation to the nucleus is impaired in L.monocytogenes infection (PubMed:20855622). .