U-Blot® Rpb1 (phospho Ser1619) Polyclonal Antibody

Name: U-Blot® Rpb1 (phospho Ser1619) Polyclonal Antibody
SKU: WP9902
Availability: InStock

$288.00

Size:50μL

SKU: WP9902-50

Catalog No.: WP9902

Specification: 50μL/100μL

Stock: In stock

U-Blot® Rpb1 (phospho Ser1619) Polyclonal Antibody Learn more

Product details Specifications Q & A Education

Background：This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008],

Product Overview

Catalog No.: WP9902
SKU: WP9902-50
Category: Polyclonal antibodies
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: Rpb1
Reactivity: Human,Mouse,Rat,Monkey
Application: WB,IHC,IF,ELISA
MW(Calculated): --
MW(Observed): 250kD
Host Species: Rabbit
Isotype: IgG
Conjugate/Modification: Phospho
Modification site: --
Recommended Dilution Ratio: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Polyclonal
Clone No.: --
Immunogen: The antiserum was produced against synthesized peptide derived from human POLR2A around the phosphorylation site of Ser1619. AA range:1585-1634
Sequence: --
Specificity: Phospho-Rpb1 (S1619) Polyclonal Antibody detects endogenous levels of Rpb1 protein only when phosphorylated at S1619.
Gene Name: POLR2A
Protein Name: DNA-directed RNA polymerase II subunit RPB1
Other Name: POLR2A;POLR2;DNA-directed RNA polymerase II subunit RPB1;RNA polymerase II subunit B1;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase III largest subunit;RNA-directed RNA polymerase II subunit RPB1
Organism-1: Human
Gene ID-1: 5430
SwissProt-1: P24928
Organism-2: Mouse
Gene ID-2: 20020
SwissProt-2: P08775
Organism-3: --
Gene ID-3: --
SwissProt-3: --
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Nucleus . Cytoplasm . Chromosome . Hypophosphorylated form is mainly found in the cytoplasm, while the hyperphosphorylated and active form is nuclear (PubMed:26566685). Co-localizes with kinase SRPK2 and helicase DDX23 at chromatin loci where unscheduled R-loops form (PubMed:28076779). .