U-Blot® B23 (phospho Thr199) Polyclonal Antibody

Name: U-Blot® B23 (phospho Thr199) Polyclonal Antibody
SKU: WP9933
Availability: InStock

$288.00

Size:50μL

SKU: WP9933-50

Catalog No.: WP9933

Specification: 50μL/100μL

Stock: In stock

U-Blot® B23 (phospho Thr199) Polyclonal Antibody Learn more

Product details Specifications Q & A Education

Background：This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009],

Product Overview

Catalog No.: WP9933
SKU: WP9933-50
Category: Polyclonal antibodies
Product Type: Other
Size: 50μL/100μL
Stock Status: In stock
Available Stock: 20
Minimum Order Quantity: 1

Storage & Compliance

Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only: Yes

Additional Specifications

Target: Nucleophosmin
Reactivity: Human,Mouse,Rat
Application: WB,IHC,IF,ELISA
MW(Calculated): --
MW(Observed): 32kD
Host Species: Rabbit
Isotype: IgG
Conjugate/Modification: Phospho
Modification site: --
Recommended Dilution Ratio: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:40000;Not yet tested in other applications.
Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source: --
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity: --
storageCondition: -15°C to -25°C/1 year(Do not lower than -25°C)
Concentration: 1 mg/ml
Clonality: Polyclonal
Clone No.: --
Immunogen: The antiserum was produced against synthesized peptide derived from human NPM around the phosphorylation site of Thr199. AA range:171-220
Sequence: --
Specificity: Phospho-B23 (T199) Polyclonal Antibody detects endogenous levels of B23 protein only when phosphorylated at T199.
Gene Name: NPM1
Protein Name: Nucleophosmin
Other Name: NPM1;NPM;Nucleophosmin;NPM;Nucleolar phosphoprotein B23;Nucleolar protein NO38;Numatrin
Organism-1: Human
Gene ID-1: 4869
SwissProt-1: P06748
Organism-2: Mouse
Gene ID-2: 18148
SwissProt-2: Q61937
Organism-3: Rat
Gene ID-3: 25498
SwissProt-3: P13084
Organism-4: --
gene ID-4: --
SwissProt-4: --
Cellular Localization: Nucleus, nucleolus . Nucleus, nucleoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis.