U-Blot® IRE1α (Phospho Ser724) Rabbit mAb

U-Blot® IRE1α (Phospho Ser724) Rabbit mAb
U-Blot® IRE1α (Phospho Ser724) Rabbit mAb
$288.00
Size:50μL
SKU: WR4932-50

Catalog No.: WR4932

Specification: 50μL/100μL

Stock: In stock

U-Blot® IRE1α (Phospho Ser724) Rabbit mAb Learn more

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Product information

Product detailsSpecificationsQ & AEducation

Background：The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq, Jul 2008],

Product Overview

Catalog No.
WR4932
SKU
WR4932-50
Category
Rabbit mAbs
Product Type
Other
Size
50μL/100μL
Stock Status
In stock
Available Stock
20
Minimum Order Quantity
1

Storage & Compliance

Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only
Yes

Additional Specifications

Target
IRE1α
Reactivity
Human,Mouse,Rat
Application
WB,IHC,IF,IP,ELISA
MW(Calculated)
110kD
MW(Observed)
110kD
Host Species
Rabbit
Isotype
IgG,Kappa
Conjugate/Modification
Phospho
Modification site
Ser724
Recommended Dilution Ratio
IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;
Form
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
--
Purification
Protein A
Purity
--
storageCondition
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
--
Clonality
Monoclonal
Clone No.
PT1195R
Immunogen
--
Sequence
--
Specificity
IRE1α (Phospho Ser724) Antibody detects endogenous levels of IRE1 protein only when phosphorylated at S724.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RHsFS
Gene Name
ERN1 IRE1
Protein Name
Serine/threonine-protein kinase/endoribonuclease IRE1
Other Name
ERN1;IRE1;Serine/threonine-protein kinase/endoribonuclease IRE1;Endoplasmic reticulum-to-nucleus signaling 1;Inositol-requiring protein 1;hIRE1p;Ire1-alpha;IRE1a
Organism-1
Human
Gene ID-1
2081
SwissProt-1
O75460
Organism-2
Mouse
Gene ID-2
78943
SwissProt-2
Q9EQY0
Organism-3
--
Gene ID-3
--
SwissProt-3
--
Organism-4
--
gene ID-4
--
SwissProt-4
--
Cellular Localization
Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:The kinase domain is activated by trans-autophosphorylation. Kinase activity is required for activation of the endoribonuclease domain.,Function:Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 KEN domain.,similarity:Contains 1 protein kinase domain.,subunit:Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of ERN1.,tissue specificity:Ubiquitously expressed. High levels observed in pancreatic tissue.,

Tech Support

ucallm

01

Documents

Datasheet, COA, SDS, and protocol files can be requested from technical support.

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Storage & Handling

Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
06

Contact Support

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Keywords:Rabbit mAbs