U-Blot® NFAT1 Rabbit mAb

U-Blot® NFAT1 Rabbit mAb
U-Blot® NFAT1 Rabbit mAb
$268.00
Size:50μL
SKU: WR4951-50

Catalog No.: WR4951

Specification: 50μL/100μL

Stock: In stock

U-Blot® NFAT1 Rabbit mAb Learn more

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Product information

Product detailsSpecificationsQ & AEducation

Background：This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012],

Product Overview

Catalog No.
WR4951
SKU
WR4951-50
Category
Rabbit mAbs
Product Type
Other
Size
50μL/100μL
Stock Status
In stock
Available Stock
20
Minimum Order Quantity
1

Storage & Compliance

Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only
Yes

Additional Specifications

Target
NFAT1
Reactivity
Human
Application
WB,IHC,IF,ELISA
MW(Calculated)
100kD
MW(Observed)
140kD
Host Species
Rabbit
Isotype
IgG,Kappa
Conjugate/Modification
Unmodified
Modification site
--
Recommended Dilution Ratio
IHC 1:100-1:500;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;
Form
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
--
Purification
Protein A
Purity
--
storageCondition
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
--
Clonality
Monoclonal
Clone No.
PT1174R
Immunogen
--
Sequence
--
Specificity
Endogenous
Gene Name
NFATC2 NFAT1 NFATP
Protein Name
Nuclear factor of activated T-cells, cytoplasmic 2 (NF-ATc2) (NFATc2) (NFAT pre-existing subunit) (NF-ATp) (T-cell transcription factor NFAT1)
Other Name
Nuclear factor of activated T-cells, cytoplasmic 2;NF-ATc2;NFATc2;NFAT pre-existing subunit;NF-ATp;T-cell transcription factor NFAT1;
Organism-1
Human
Gene ID-1
4773
SwissProt-1
Q13469
Organism-2
Mouse
Gene ID-2
18019
SwissProt-2
Q60591
Organism-3
--
Gene ID-3
--
SwissProt-3
--
Organism-4
--
gene ID-4
--
SwissProt-4
--
Cellular Localization
Alternative products:Additional isoforms seem to exist,Domain:Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.,Function:Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF.,induction:Inducibly expressed in T-lymphocytes upon activation of the T-cell receptor (TCR) complex. Induced after co-addition of phorbol 12-myristate 13-acetate (PMA) and ionomycin.,PTM:In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.,similarity:Contains 1 RHD (Rel-like) domain.,subcellular location:Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.,subunit:Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. The phosphorylated form specifically interacts with XPO1; which mediates nuclear export. NFATC proteins bind to DNA as monomers. Interacts with NFATC2IP.,tissue specificity:Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas.,

Tech Support

ucallm

01

Documents

Datasheet, COA, SDS, and protocol files can be requested from technical support.

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Storage & Handling

Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
06

Contact Support

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Keywords:Rabbit mAbs