U-Blot® Bcl-2 (Phospho Ser70) Rabbit mAb

U-Blot® Bcl-2 (Phospho Ser70) Rabbit mAb
U-Blot® Bcl-2 (Phospho Ser70) Rabbit mAb
$288.00
Size:50μL
SKU: WR5102-50

Catalog No.: WR5102

Specification: 50μL/100μL

Stock: In stock

U-Blot® Bcl-2 (Phospho Ser70) Rabbit mAb Learn more

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Product information

Product detailsSpecificationsQ & AEducation

Background：BCL2, apoptosis regulator(BCL2) Homo sapiens This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016],

Product Overview

Catalog No.
WR5102
SKU
WR5102-50
Category
Rabbit mAbs
Product Type
Other
Size
50μL/100μL
Stock Status
In stock
Available Stock
20
Minimum Order Quantity
1

Storage & Compliance

Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Research Use Only
Yes

Additional Specifications

Target
Bcl-2 Phospho Ser70
Reactivity
Human
Application
WB,IF,IP,ELISA
MW(Calculated)
26kD
MW(Observed)
26kD
Host Species
Rabbit
Isotype
IgG,Kappa
Conjugate/Modification
Phospho
Modification site
Ser70
Recommended Dilution Ratio
WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;
Form
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
--
Purification
Protein A
Purity
--
storageCondition
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
--
Clonality
Monoclonal
Clone No.
PT1385R
Immunogen
--
Sequence
--
Specificity
Bcl-2 (Phospho Ser70) Antibody detects endogenous levels of Bcl-2 protein only when phosphorylated at S70.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RTsPL
Gene Name
BCL2
Protein Name
Apoptosis regulator Bcl-2
Other Name
BCL2;Apoptosis regulator Bcl-2
Organism-1
Human
Gene ID-1
596
SwissProt-1
P10415
Organism-2
Mouse
Gene ID-2
12043
SwissProt-2
P10417
Organism-3
Rat
Gene ID-3
24224
SwissProt-3
P49950
Organism-4
--
gene ID-4
--
SwissProt-4
--
Cellular Localization
Disease:A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.,Domain:The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1.,Function:Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).,online information:Bcl-2 entry,PTM:Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A).,PTM:Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.,similarity:Belongs to the Bcl-2 family.,subunit:Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (By similarity). Also interacts with APAF1, RAF-1, TP53BP2, BBC3, BCL2L1, MRPL41 and BNIPL. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets.,tissue specificity:Expressed in a variety of tissues.,

Tech Support

ucallm

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Documents

Datasheet, COA, SDS, and protocol files can be requested from technical support.

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Storage & Handling

Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
06

Contact Support

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Keywords:Rabbit mAbs