- Target
- --
- Reactivity
- Human, Wide Range Predicted
- Applications
- CUT&Tag; ChIP; ChIP-Seq; WB
- MW(Calculated)
- 17 kDa
- Host Species
- Rabbit
- Isotype
- IgG
- Recommended Dilution Ratio
- Applications Validated by Active Motif: ChIP-Seq: 5 µg each WB*: 0.5 - 2 µg/ml dilution HPA: 1:20000 dilution. CUT&Tag: 1-2 µg per 50 µl reaction *For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.05% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.
- Formulation
- Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
- Purification
- Protein A Chromatography
- Storage
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
- Concentration
- --
- Clonality
- Recombinant
- Clone Number
- --
- Immunogen
- This antibody was raised against a peptide containing mono-methyl lysine 4 of Histone H3.
- Specificity
- --
- Gene Name
- --
- Other Name
- --
- Gene ID
- --
- Cellular Localization
- --
- Function
- --