U-Blot® Methylated-Histone H3 (H3K4me3) and Histone H3 ELISA Kit

The Methylated-Histone H3 (H3K4me3) and Histone H3 ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human, mouse and rat cell lysates. By determining Methylated Histone H3 (H3K4me3) protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human, mouse and rat Methylated-Histone H3 (H3K4me3). An anti-pan Histone H3 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Histone H3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-Methylated-Histone H3 antibody is used to detect Methylated Histone H3 (H3K4me3). After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Methylated Histone H3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.Notably, this kit allows simultaneous measurement of methylated protein and pan protein in a single experiment for accurate data normalization. It supports high-throughput screening of numerous different cell lysates without the time-consuming and labor-intensive Western Blot analysis. Featuring minimal hands-on operation time, simple workflow, and a completely non-radioactive detection system, this assay provides a convenient and safe solution for histone methylation research.