U-Blot® Human Protein Nitration Antibody Array G1

Protein nitration plays an unusually prominent role in cell signaling, development, and growth. The Human Protein Nitration Antibody Array 1 is a very rapid, convenient, and sensitive assay that can simultaneously detect multiple tyrosine nitration events on proteins. It can also be used to monitor the activation or function of important biological pathways. The Human Protein Nitration Antibody Array 1 is specifically designed for the simultaneous identification of the relative levels of tyrosine nitration in 507 different human proteins in cell lysate. By monitoring changes in protein nitration in your experimental model system, you can verify pathway activation in your cell lines without the need for extensive time and effort required for immunoprecipitation and/or Western Blot analysis. To use the Human Protein Nitration Antibody Array 1, treated or untreated cell lysate is added to the antibody array glass slide wells. The antibody array slide wells are then washed, and a biotinylated anti-nitrated-tyrosine antibody is used to detect the nitrated tyrosine residues on target proteins. After incubation with a fluorescent dye-conjugated streptavidin (Cy3 equivalent), the slides can be imaged using a laser scanner, such as the Axon GenePix, in the Cy3 channel.