U-Blot® Human Phosphorylation Pathway Profiling Array C55

U-Blot® Human Phosphorylation Pathway Profiling Array C55
U-Blot® Human Phosphorylation Pathway Profiling Array C55
$9,999.00
SKU: WT9607A-C55-4

Specification: 4 Sample Kit

Stock: In stock

Product details, specifications, documents, and related products are available from the catalog record. Learn more

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U-Blot® Human Phosphorylation Pathway Profiling Array C55

Product Overview

Catalog No.
WT9607A-C55-4
SKU
WT9607A-C55-4
Category
Phosphorylation
Product Type
Other
Size
4 Sample Kit
Stock Status
In stock
Available Stock
20
Minimum Order Quantity
1

Storage & Compliance

Research Use Only
Yes

Additional Specifications

Size
4 Sample Kit
Species
Human
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-Quantitative
Semi-Quantitative
Number of Targets Detected
55
Solid Support
Membrane
Design Principle
Sandwich-based
Method Of Detection
Chemiluminescence
Research Area
Post-Translational Modifications, Phosphorylation, JAK/STAT Signaling, MAPK Signaling, PI3K-AKT Signaling, TGF-beta Signaling, NFkB Signaling, Akt Signaling
Introduction
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Product Features
Easy to use;No specialized equipment needed;Compatible with nearly any liquid sample;Proven technology (many publications);Highly sensitive (pg/ml);Sandwich ELISA specificity;Higher density than ELISA, Western blot or bead‑based multiplex
Suggested Application
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Kit Components
Human Phosphorylation Pathway Profiling Array C55 Membranes;Blocking Buffer;Detection Antibody Cocktail;500X HRP‑Anti‑Rabbit IgG Concentrate;20X Wash Buffer I Concentrate;20X Wash Buffer II Concentrate;2X Cell Lysis Buffer Concentrate;Detection Buffer C;Detection Buffer D;8‑Well Incubation Tray w/ Lid;Protease Inhibitor Cocktail;100x Phosphatase Inhibitor Cocktail I;Phosphatase Inhibitor Cocktail II;Plastic Sheets;Array Map Template;Manual
Other Materials Required
Pipettors, pipet tips and other common lab consumables;Orbital shaker or oscillating rocker;Tissue Paper, blotting paper or chromatography paper;Adhesive tape or Saran Wrap;Distilled or de‑ionized water;A chemiluminescent blot documentation system (such as UVP's ChemiDoc‑It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X‑ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
1.Block membranes;2. Incubate with Sample;3. Incubate with Detection Antibody Cocktail;4. Incubate with HRP‑Conjugated anti‑IgG;5. Incubate with Detection Buffers;6. Image with chemiluminescent imaging system;7. Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Tech Support

ucallm

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Documents

Datasheet, COA, SDS, and protocol files can be requested from technical support.

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Contact Support

Emailinfo@mail.ucallm.com

Phone+(1)-866-986-9598

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HoursMonday-Friday 09:00-18:00 CST

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Keywords:Phosphorylation