U-Blot® Human Protein Lactylation Array G8000

U-Blot® Human Protein Lactylation Array G8000
U-Blot® Human Protein Lactylation Array G8000
$9,999.00
SKU: WT9610A-G8000-4

Specification: 4 Sample Kit

Stock: In stock

Product details, specifications, documents, and related products are available from the catalog record. Learn more

Product information

Protein Lactylation plays an unusually prominent role in cell signaling, development and growth. The G‑Series Human Protein Lactylation Antibody Array 8000 is a very rapid, convenient, and sensitive assay that can simultaneously detect multiple protein Lactylation of lysine and be used to monitor the activation or function of important biological pathways. The Human Protein Lactylation Antibody Array 8000 is specifically designed for simultaneous identification of the relative levels of Lactylation of lysine of 8000 different Human Proteins in cell lysate. By monitoring the changes in protein Lactylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort performing an analysis of immunoprecipitation and/or Western Blot. To use the G‑Series Human Protein Lactylation Antibody Array 8000, treated or untreated cell lysate is added into antibody array glass slide wells. The antibody array slide wells are washed, and a biotinylated anti‑Lactyllysine antibody is then used to detect the lactylated‑lysine on target proteins. After incubation with a fluorescent dye‑conjugated streptavidin (Cy3 equivalent), the slides can then be imaged using a laser scanner, such as the Axon GenePix, using the Cy3 channel.

Product Overview

Catalog No.
WT9610A-G8000-4
SKU
WT9610A-G8000-4
Category
Lactylation (L/D-Lactylation)
Product Type
Other
Size
4 Sample Kit
Stock Status
In stock
Available Stock
20
Minimum Order Quantity
1

Storage & Compliance

Research Use Only
Yes

Additional Specifications

Size
4 Sample Kit
Species
Human
Compatible Sample Types
Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-Quantitative
Semi-Quantitative
Number of Targets Detected
8000
Solid Support
Glass Slide
Design Principle
Sandwich-based
Method Of Detection
Fluorescence Laser Scanner
Research Area
Post-Translational Modifications, Epigenetics, Lactylation
Introduction
Protein Lactylation plays an unusually prominent role in cell signaling, development and growth. The G‑Series Human Protein Lactylation Antibody Array 8000 is a very rapid, convenient, and sensitive assay that can simultaneously detect multiple protein Lactylation of lysine and be used to monitor the activation or function of important biological pathways. The Human Protein Lactylation Antibody Array 8000 is specifically designed for simultaneous identification of the relative levels of Lactylation of lysine of 8000 different Human Proteins in cell lysate. By monitoring the changes in protein Lactylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort performing an analysis of immunoprecipitation and/or Western Blot. To use the G‑Series Human Protein Lactylation Antibody Array 8000, treated or untreated cell lysate is added into antibody array glass slide wells. The antibody array slide wells are washed, and a biotinylated anti‑Lactyllysine antibody is then used to detect the lactylated‑lysine on target proteins. After incubation with a fluorescent dye‑conjugated streptavidin (Cy3 equivalent), the slides can then be imaged using a laser scanner, such as the Axon GenePix, using the Cy3 channel.
Product Features
High sensitivity and specificity;Low sample volume (10-100 µl per array);Large dynamic range of detection;Compatible with most sample types;Test 4 or 8 samples on each slide;Suitable for high-throughput assays
Suggested Application
--
Kit Components
Human Antibody Array Slide(s);Blocking Buffer;Biotinylated Detection Antibody;Fluorescent Dye‑Conjugated Streptavidin;Wash Buffer I;Wash Buffer II;Wash Buffer III;Lysis Buffer;Protease Inhibitor Cocktail;Adhesive film
Other Materials Required
Shaker;Laser scanner for fluorescence detection;Aluminum foil;Distilled water;Plastic box;50 mL Centrifuge tube;Isopropanol (2‑propanol)
Protocol Outline
Dry the glass slide;Add Blocking Buffer to each well and incubate.;Add the sample to each well and incubate.;Wash the wells.;Add Biotinylated Detection Antibody solution to each well and incubate.;Add Fluorescent Dye‑Conjugated Streptavidin solution to each well and incubate.;Scan with a gene microarray laser scanner;Perform densitometry and analysis
Storage/Stability
Store kit at ≤ -20°C immediately upon arrival. Kit must be used within the 6 months expiration date.

Tech Support

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Documents

Datasheet, COA, SDS, and protocol files can be requested from technical support.

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Keywords:Lactylation (L/D-Lactylation)